Amended Snags article
نویسندگان
چکیده
1 The unusual behavior during purification of four affinity-tagged proteins—a MAP kinase, a transcription factor, intracellular binding protein, and a receptor tyrosine kinase—led the authors to devise alternate methods of obtaining their end-product. They found it necessary to either remove the affinity tag (by recloning or by cleavage of the tag) or omit the traditional immobilized metal ion adsorption chromatography (IMAC) step altogether from the purification scheme.
منابع مشابه
Characteristics of snags containing excavated cavities in northern Arizona mixed-conifer and ponderosa pine forests
Snags provide an important resource for a rich assemblage of cavity-nesting birds in the southwestern United States. To expand our knowledge of snag use by cavity-nesting birds in this region, we documented characteristics of snags with and without excavated cavities in mixed-conifer and ponderosa pine (Pinus ponderosa Dougl. ex Laws) forest in north-central Arizona. Snags were sampled in 113 s...
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We compared the density of snags, snags with cavities, and cavity-nesting bird use at two sites in northern California: Blacks Mountain Experimental Forest, a site with large trees and large snags because of protection from logging, contrasted with the Goosenest Adaptive Management Area, where a century of logging left this forest with few large trees and snags. Indeed, there was a threefold di...
متن کاملSnag longevity in relation to wildfire and postfire salvage logging
Snags create nesting, foraging, and roosting habitat for a variety of wildlife species. Removal of snags through postfire salvage logging reduces the densities and size classes of snags remaining after wildfire. We determined important variables associated with annual persistence rates (the probability a snag remains standing from 1 year to the next) of large conifer snags ( 23 cm diameter brea...
متن کاملDecay of Fire-caused Snags in Ocala San Ine
Sand pine (I-‘inu.r c/trrr.srr) scrub is adapted to, and regenerated by, periodic stantl-rcplnccment wildfire, which consumch the understory and kills the overstay. The heat of the fire opens the serotinous cones of’ Ocala sand pine (P. c~/nu.srr var. c/tr~c.sc/), releasing quantities of seed that reestablish the overstory, while the understory regenerates by sproutin, (1 or from soil-stored se...
متن کاملFall-down Rates of Subalpine Fir Snags at Sicamous Creek: Implications for Worker Safety and Habitat Supply
Current regulations require that all dead trees over m tall are felled when an area is being logged (Workers’ Compensation Board regulation .). This clearly protects workers because tree-falling, especially handfalling, is more dangerous when other trees, live or dead, could interfere with the falling tree. Snags must also be removed within . tree heights of work areas. This buffer is i...
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